Procell system

Hela Cell Line

Product Code:
CL-0101
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
1 / 1
❮❯

No additional charges, what you see is what you pay! *

Show prices   How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: China.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • References
  • Related Products
  • Show All

Further Information

Age Gender:
31 years adult
Background:
The cells are positive for keratin by immunoperoxidase staining.HeLa cells have been reported to contain human papilloma virus 18(HPV-18) sequences.P53 expression was reported to be low, and normal levels of pRB(retinoblastoma suppressor) were found.
Biosafety Level:
BSL-2
Cell Type:
Cancer cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
MEM(PM150410)+10% FBS+1% P/S(PB180120)
Doubling Time:
~32-48 hours
Gene expression:
Lysophosphatidylcholine(Lyso-Pc) Induces Ap-1 Activity And C-Jun N-Terminal Kinase Activity(Jnk1) By A Protein Kinase C-Independent Pathway. The Cells Are Positive For Keratin By Immunoperoxidase Staining.
Growth Properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:2-1:4
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Uterus; Cervix

Documents

References

  • https://www.nature.com/articles/s41591-022-02194-3
  • https://www.nature.com/articles/s41565-024-01683-5
  • https://www.nature.com/articles/s41565-021-00952-x