Procell system

JeKo-1 Cell Line

Product Code:
CL-0128
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
78 years
Antigen expression:
CD3-, CD5+, CD10+, CD19+
Applications:
JeKo-1 cells are highly tumorigenic in SCID mice.
Background:
The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. Ref JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
RPMI-1640(PM150110)+20% FBS+1% P/S(PB180120)
Doubling Time:
~26-50 hours
Gene expression:
Cd3-, Cd5+, Cd10+, Cd19+
Growth Properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Lymphoblast-like
Split Ratio:
3x10^5-2x10^6 cells/mL
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2?10^5 viable cells/ml. Maintain cell density between 1?10^5 and 1?10^6 viable cells/ml.
Tissue:
Peripheral blood

Documents

References

  • https://ashpublications.org/bloodadvances/article/doi/10.1182/bloodadvances.2023012202/515576
  • https://www.sciencedirect.com/science/article/pii/S0223523422009060
  • https://www.embopress.org/doi/abs/10.1038/s44319-025-00385-w