K-562 [K562] Cell Line

53 years

CD7(25%)

Transfection.

The cell population has been characterized as highly undifferentiated and of the granulocytic series. Studies conducted by Anderson, et al. , on the surface membrane properties led to the conclusion that the K-562 was a human erythroleukemia line. K-562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognizable progenitors of the erythrocytic, granulocytic and monocytic series. The effect of inducers on sublines derived from the original K-562 cell line have been reviewed by Koeffler and Golde. Karyological studies on various K-562 sublines have been classified into three groups(A,B,C) by Dimery, et al. The strain obtained by the ATCC most closely resembles the B population. Occurrence of the Philadelphia chromosome, however, was of much lower frequency; none detected in 15 metaphases examined. The line is EBNA negative.

BSL-1

Cancer cell line

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

IMDM(PM150510)+10% FBS+1% P/S(PB180120)

~30-48 hours

Yes, in nude mice. Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.

Suspension

Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C

37°C

Every 2 to 3 days

Lymphoblast-like

3x10^5-5x10^5 cells/mL

1:2 to 1:4

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.

Bone Marrow

Yes