Elabscience

KLE cell line

Product Code:
CL-0133
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
female, 64 years
Antigen expression:
Blood Type O; Rh+
Background:
The KLE cell line was established by G. R. Richardson in 1984 from endometrial tumor tissue in a 64-year-old female Caucasian. The chromosome of KLE cells is aneuploid, with multiple chromosome numbers in the range of 51-66. Electron microscopy of tumors formed in nude mice shows microvilli and junctional complexes, and nucleolar channel systems are present that are similar to those seen in normal endometrium under progestational stimulation.
Biosafety Level:
1
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
DMEM/F12(PM150312)+10% FBS+1% P/S(PB180120)
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Medium Renewal:
2 times per week
Morphology:
Epithelial
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Uterus, Endometrium
Tumorigenic:
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1?10^7 cells.

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