Elabscience

LoVo Cell Line

Product Code:
CL-0144
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

No additional charges, what you see is what you pay! *

Show prices   How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: China.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Related Products
  • Show All

Further Information

Age Gender:
56 years
Antigen expression:
HLA A11, B15, B17, Cw1, Cw3; Blood Type B
Applications:
Transfection host.
Background:
The cells are negative for expression of CSAp(CSAp-) and colon antigen 3.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.N-myc and sis oncogene expression were not detected.Tumor specific nuclear matrix proteins CC-3 and CC-4 are expressed.
Biosafety Level:
1
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
Ham's F-12K(PM150910)+10% FBS+1% P/S(PB180120)
Effects:
Yes, in nude mice.
Gene expression:
Carcinoembryonic Antigen(Cea) 908Ng/10^6 Cells/10Days. The Cells Are Negative For Expression Of Csap(Csap-) And Colon Antigen 3.
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Epithelial
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Colon, derived from metastatic site: left supraclavicular region
Tumorigenic:
Yes

Documents