Procell system

MOLT-4 Cell Line

Product Code:
CL-0160
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
19 years
Applications:
Transfection.
Background:
The patient had received prior multidrug chemotherapy.There is a G- A mutation at codon 248 of the p53 gene.P53 is not expressed.The cells do not produce immunoglobulin or Epstein-Barr virus.The line was originally contaminated with mycoplasma but has been cured with antibiotics.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Doubling Time:
~24-48 hours
Effects:
Yes, in untreated nude mice, anti lymphocyte serum treated mice and X-irradiated mice.
Gene expression:
High Levels Of Terminal Deoxynucleotidyl Transferase(Tdt) Are Produced.
Growth Properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Lymphoblast-like
Split Ratio:
4x10^5 cells/mL
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2?10^5 viable cells/ml. Maintain cell density between 1?10^5 and 1?10^6 viable cells/ml.
Tissue:
Peripheral blood
Tumorigenic:
Yes

Documents

References

  • https://pmc.ncbi.nlm.nih.gov/articles/PMC11647336/
  • https://www.sciencedirect.com/science/article/pii/S174801322400392X
  • https://www.sciencedirect.com/science/article/pii/S1043661823000427