PK-15 Cell Line

Adult

Transfection host.

The presence of a porcine papovavirus in PK-15 cells has been reported in cells obtained from multiple sources including the ATCC. The Foreign Animal Disease Diagnostic Laboratory of the US Department of Agriculture has determined that ATCC CCL-33 is not infected with Hog cholera virus or African swine fever virus, and uses this line to screen for those viruses. The cell line harbors an endogenous C-type retrovirus. The cells are positive for porcine circovirus(PCV) antigens. The cells are positive for keratin by immunoperoxidase staining.

BSL-1

spontaneously immortalized cell line

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

MEM(PM150467)+10% FBS+1% P/S(PB180120)

~12-25 hours

Adherent

Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C

37°C

Every 2 to 3 days

Epithelial-like

1:2-1:4

1:2 to 1:4

Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

kidney