Elabscience

RAW 264.7 Cell Line

Product Code:
CL-0190
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Mouse
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
Adult
Applications:
This cell line is a suitable transfection host. The cells will pinocytose neutral red and will phagocytose latex beads and zymosan. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets. LPS or PPD treatment for 2 days
Background:
This cell line is easy to propagate, high efficiency for DNA transfection, sensitivity to RNA interference, and supports replication of murine noroviruses. This cell line is negative for surface immunoglobulin(sIg-), Ia(Ia-) and Thy-1.2(Thy-1.2). When this line was established, it was described as not secreting detectable virus particles and negative using the XC plaque formation assay. Based on a published study by Dr. Janet W. Hartley in 2008, this line was demonstrated to express ecotropic and polytropic MuLV, and is positive using the XC plaque assay for virus replication.Ref
Biosafety Level:
2
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
DMEM(PM150210)+10% FBS+1% P/S(PB180120)
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Monocyte/macrophage
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Subcultures are prepared by scraping or blowing. For a 100mm disk,remove all but 10 mL culture medium (adjust amount accordingly for otherculture vessels). Dislodge cells from the flask substrate with a cell scraperor blowing directly with the pipettes; aspirate and add appropriatealiquots of the cell suspension into new culture vessels.
Tissue:
Abelson murine leukemia virus-induced tumor, ascites

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