Procell system

A-498 cell line

Product Code:
CL-0254
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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Further Information

Age Gender:
female, 52 years
Background:
The A-498 cell line was established by S. Aaronson from a 52-year-old female patient with kidney cancer.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
MEM(PM150410)+10% FBS+1% P/S(PB180120)
Doubling Time:
~60 hours
Growth Properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
2 times per week
Morphology:
Epithelial-like
Split Ratio:
1:2-1:4
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
kidney
Tumorigenic:
Yes, in nude mice; forms undifferentiated carcinoma; also forms tumors in anti thymocyte serum treated newborn mice.

Documents

References

  • https://www.nature.com/articles/s41418-026-01723-8
  • https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202100881
  • https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202401142