Elabscience

D283 Med Cell Line

Product Code:
CL-0271
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
6 years juvenile
Background:
The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein.
Biosafety Level:
1
Cryopreservation:
Freeze Medium: 55% Basal Medium+40% FBS+17% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
MEM (PM150410)+10% FBS+1% P/S (PB180120)
Effects:
Yes, in nude mice(The cells form serially transplantable intercranial and subcutaneous tumors.). Yes, in soft agar.
Gene expression:
The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells
Growth properties:
Suspension, multicell aggregates and some adherent cells
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial
Subcultivation Ratio:
2?10^5 to 5?10^5cells/mL
Tissue:
Brain/cerebellum, derived from metastatic site: peritoneum
Tumorigenic:
Yes

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