Procell system

D283 Med Cell Line

Product Code:
CL-0271
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
6 years juvenile
Background:
The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze Medium: 55% Basal Medium+40% FBS+17% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
MEM (PM150410)+10% FBS+1% P/S (PB180120)
Doubling Time:
~52 hours
Effects:
Yes, in nude mice(The cells form serially transplantable intercranial and subcutaneous tumors.). Yes, in soft agar.
Gene expression:
The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells
Growth Properties:
Semi-adherent and semi-suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:2-1:4
Subcultivation Ratio:
2?10^5 to 5?10^5cells/mL
Tissue:
Cerebellum; derived from metastatic site: ascites
Tumorigenic:
Yes

Documents

References

  • https://academic.oup.com/noa/advance-article-abstract/doi/10.1093/noajnl/vdag109/8665122
  • https://www.sciencedirect.com/science/article/pii/S0967586821000291