MDA-MB-468 Cell Line

51 years

Blood Type AB; HLA Aw23, Aw30, B27, Bw35, Cw2, Cw4(patient)

Transfection host.

Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype. There is a G- A mutation in codon 273 of the p53 gene resulting in an Arg- His substitution. EGF receptor is present at 1?10^6 per cell.

BSL-1

Cancer cell line

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Leibovitz's L-15(PM151010)+10% FBS+1% P/S(PB180120)

~36-62 hours

Yes, in nude mice inoculated subcutaneously with 10^7 cells. Tumors developed within 21 days at 100% frequency(5/5).

Adherent

Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C

37°C

Every 2 to 3 days

Epithelial-like

Epidermal growth factor(EGF); transforming growth factor alpha(TGF alpha)

1:2-1:4

1:2 to 1:4

Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Mammary gland/breast; derived from metastatic site: pleural effusion

Yes