Elabscience

SW1116 Cell Line

Product Code:
CL-0307
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
73 years
Antigen expression:
Blood Type O; Rh+
Background:
CSAp negative(CSAp-).Colon antigen 3, negative.The cells are positive for keratin by immunoperoxidase staining.The line is positive for expression of c-myc, K-ras, H-ras, myb, sis and fos oncogenes.N-myc and N-ras oncogene expression were not detected.Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.
Biosafety Level:
1
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
Leibovitz's L-15(PM151010)+10% FBS+1% P/S(PB180120)
Effects:
Yes, in nude mice. Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
Gene expression:
Carcinoembryonic Antigen(Cea) 2654Ng/10^6 Cells/10 Days; Keratin
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Epithelial
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Cells must be subcultured at about 80% confuency , before they reach 90%. Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Colon
Tumorigenic:
Yes

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