Procell system

BT-20 Cell Line

Product Code:
CL-0324
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
74 years
Antigen expression:
Antigen expression: HLA A1, Bw16 (+/-)
Background:
The cells express the WNT3 and the WNT7B oncogenes . Growth of BT-20 cells is inhibited by tumor necrosis factor alpha (TNF alpha). BT-20 cells are negative for estrogen receptor, but do express an estrogen receptor mRNA that has deletion of exon 5.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze Medium: 55% Basal Medium+40% FBS+21% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
MEM (PM150410)+10% FBS+1% P/S (PB180120)
Doubling Time:
~70 hours
Effects:
Yes, in nude mice(The cells form grade II adenocarcinomas.).
Growth Properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:2-1:4
Subcultivation Ratio:
1:2 to 1:4
Tissue:
Breast
Tumorigenic:
Yes

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S0304383524002350
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10437062/
  • https://www.nature.com/articles/s41598-023-49136-8