Procell system

J774A.1 Cell Line

Product Code:
CL-0370
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Mouse
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
Adult
Applications:
Biological response. Transfection host
Background:
J774A. 1 cells are active in antibody dependent phagocytosis. Their growth is inhibited by dextran sulfate, PPD and LPS. They synthesize large amounts of lysozyme and exhibits minor cytolysis but predominantly antibody-dependent phagocytosis. Interleukin 1 beta(Il1b) is synthesized continuously by this line.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
DMEM(PM150210)+10% FBS+1% P/S(PB180120)
Doubling Time:
~17 hours
Growth Properties:
Adherent, trypsin-free for passaging
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Monocytic; macrophage-like
Receptor expression:
Complement(C3), expressed; Fc receptor, IgG, high affinity I(Fcgr1), expressed
Split Ratio:
1:2-1:4
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Subcultures are prepared by scraping or blowing. For a 100mm disk,remove all but 10 mL culture medium (adjust amount accordingly for otherculture vessels). Dislodge cells from the flask substrate with a cell scraperor blowing directly with the pipettes; aspirate and add appropriatealiquots of the cell suspension into new culture vessels.
Tissue:
Ascites

Documents

References

  • https://www.nature.com/articles/s41423-025-01292-9
  • https://pubs.acs.org/doi/abs/10.1021/acsnano.3c08097
  • https://www.cell.com/trends/biotechnology/abstract/S0167-7799(26)00050-8