Procell system

K7M2 wt [K7M2-WT] Cell Line

Product Code:
CL-0371
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Mouse
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
Adult
Applications:
Biological response. Transfection host
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
DMEM(PM150210)+10% FBS+1% P/S(PB180120)
Doubling Time:
~31 hours
Effects:
Yes, forms tumors in BALB/c mice with spontaneous metastasis to the lungs in over 90% of mice inoculated.
Growth Properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Osteoblast-like
Receptor expression:
Complement(C3), expressed; Fc receptor, IgG, high affinity I(Fcgr1), expressed
Split Ratio:
1:2-1:4
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Bone; derived from metastatic site: lung
Tumorigenic:
Yes

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S1385894724023088
  • https://www.nature.com/articles/s41467-025-61377-x
  • https://jeccr.biomedcentral.com/articles/10.1186/s13046-022-02372-8