Elabscience

MDA-MB-415 Cell Line

Product Code:
CL-0382
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
38 years
Antigen expression:
Blood Type O
Background:
The cells express the WNT7B oncogene [PubMed: 8168088].The patient presenting with the tumor was from Paraguay and, although listed as Caucasian, may have had mixed ancestry as suggested by the presence of G6PD type A phenotype in the cells.The line forms flat, spreading plaques of epithelia which exhibit desmosomes, extensive microtubules and microfilaments upon examination with the electron microscope. It is not amenable to trypsinization.
Biosafety Level:
1
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
Leibovitz's L-15(PM151010)+10ug/ml Insulin+10ug/ml Glutathione+15% FBS+1% P/S(PB180120)
Effects:
Yes, in semisolid medium; No, in immunosuppressed mice.
Gene expression:
Amelogenin(X Chromosome)(Amelex)
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Epithelial
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Mammary gland/breast, derived from metastatic site: pleural effusion
Tumorigenic:
No

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