NCI-H446 [H446] cell line

male, 61 years

This cell line was established in 1982 by D. Carney, A.F. Gazdar and associates from the pleural fluid of a patient with small cell cancer of the lung. The original tumor morphology was not characteristic of small cell lung cancer. The cell line is a variant small cell lung cancer in biochemistry and morphology, and expresses neuron specific enolase as well as the brain isoenzyme of creatine kinase. None of L-DOPA decarboxylase, bombesin, vasopressin, oxytocin or gastrin releasing peptide has been detected in the cell line. This cell line exhibits a 20-fold higher degree of c-myc DNA amplification and a 15-fold higher degree of c-myc RNA. The cell line was originally propagated in serum free RPMI 1640 medium supplemented with 10 nM of hydrocortisone, 5 ?g/mL of insulin, 10 ?g/mL of transferrin, 10 nM of 17-beta-estradiol, and 30 nM of sodium selenite. Transplantable tumors with non-typical small cell lung cancer histology can be formed by the cells.

BSL-1

Cancer cell line

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Semi-adherent and semi-suspension

Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C

37°C

2 to 3 times per week

Epithelial-like

1:2-1:4

1:3 to 1:4

Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.

Lung; derived from metastatic site: pleural effusion

Yes, in nude mice (The cells form transplantable tumors with non-typical SCLC histology).