Procell system

PC-12 Cell Line (Undifferentiated)

Product Code:
CL-0412
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Rat
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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  • Further Information
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Further Information

Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze Medium: 55% Basal Medium+40% FBS+34% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
RPMI-1640(PM150110)+15%HS+5% FBS+1% P/S(PB180120)
Growth Properties:
Aggregated suspension with loose adherence
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Round
Split Ratio:
1:2-1:4
Subcultivation Ratio:
2?10^5 to 5?10^5cells/mL
Subculturing:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Tissue:
Adrenal gland

Documents

References

  • https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/adma.202415053
  • https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/adma.202512404
  • https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/adma.73023