Procell system

Sp2/0-Ag14 cell line

Product Code:
CL-0445
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Mouse
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Antigen expression:
H-2d
Background:
This cell line is a non-immunoglobulin-secreting line created by fusing a BALB/c mouse spleen cell with the mouse myeloma P3X63Ag8. The cells are resistant to 8-azaguanine (20 ug/mL) but sensitive to HAT. This cell line was tested for being not infected by ectromelia virus (mousepox).
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
DMEM(PM150210)+10% FBS+1% P/S(PB180120)
Growth Properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Replace half of the media every 2 to 3 days
Morphology:
Lymphoblast-like
Split Ratio:
3x10^5-5x10^5 cells/mL
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Tissue:
Spleen

Documents

References

  • https://www.cell.com/cell-reports-medicine/fulltext/S2666-3791(25)00284-8
  • https://www.degruyter.com/document/doi/10.1515/nanoph-2020-0150/html
  • https://pmc.ncbi.nlm.nih.gov/articles/PMC12467161/