Procell system

TM4 Cell Line

Product Code:
CL-0456
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Mouse
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
11 to 13 days
Antigen expression:
H-Y antigen; Mus musculus, expressed
Background:
The TM4 cell line is reported to respond to FSH with an increase in cAMP production, but to not respond to luteinizing hormone(LH). The FSH responsiveness is much reduced compared to primary sertoli cell cultures. Constitutive plasminogen activator production is reported to be low, but is stimulated by FSH and, to a greater extent, by retinoic acid.Tested and found negative for ectromelia virus(mousepox).
Biosafety Level:
BSL-1
Cell Type:
spontaneously immortalized cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture medium:
DMEM/F12(PM150312)+5% HS+2.5% FBS+1% P/S(PB180120)
Effects:
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Gene expression:
Retinol Binding Protein, Tissue Plasminogen Activator, Transferrin
Growth Properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Epithelial-like
Receptor expression:
Follicle stimulating hormone(FSH), expressed; androgen receptor, expressed; progesterone receptor, expressed
Split Ratio:
1:3-1:8
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Testis
Tumorigenic:
No

Documents

References

  • https://www.nature.com/articles/s41467-022-34990-3
  • https://pubs.acs.org/doi/abs/10.1021/acsnano.4c15112
  • https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/advs.202522278