Procell system

UMR-106 Cell Line

Product Code:
CL-0462
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Rat
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Background:
The cells are responsive to PTH, prostaglandins and bone resorbing steroids.The PTH responsiveness of UMR-106 is greater than that of the related cell line UMR-108.Activation of protein kinase C inhibits ATP induced increases in intracellular calcium levels.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
DMEM(PM150210)+10% FBS+1% P/S(PB180120)
Growth Properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Epithelial-like
Receptor expression:
Parathyroid hormone(PTH); 1-25(OH)2D3(bone resorbing steroid hormone)
Split Ratio:
1:3-1:6
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Bone

Documents

References

  • https://pubs.acs.org/doi/abs/10.1021/acsami.5c16825
  • https://www.sciencedirect.com/science/article/pii/S2214031X25000804
  • https://www.sciencedirect.com/science/article/pii/S0944711324000953