Procell system

Super Tube cell line

Product Code:
CL-0473
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Canine (Dog)
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
female, adult
Background:
Super Tube is an epothelial-like cell line cloned from the MDCK, a cell line from an adult cocker spaniel, Canis familiaris. Compared to the original cell line, Super Tube expresses a much lower cyclic-AMP resting level and responces less readily to forskolin stimulation of adenylate cyclase. Additionally, Super Tube shows a lower transepithelal resistance compared to Super Dome and MDCK.
Biosafety Level:
BSL-1
Cell Type:
spontaneously immortalized cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
DMEM/F12(PM150312)+0.05mM NEAA+10% FBS+1% P/S(PB180120)
Growth Properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:3-1:4
Subcultivation Ratio:
1:3 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
kidney

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