Procell system

Ramos [RA 1] Cell Line

Product Code:
CL-0483
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
3 years
Antigen expression:
CD23+
Applications:
Transfection.
Background:
The cells are negative for Epstein-Barr virus. Both the parental line and the derivative cell line Ramos. 2G6. 4C10 have about 1500 IL-4 binding sites per cell. The cells are reported to secrete IgM(lambda light chain).
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture medium:
RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Doubling Time:
~48 hours
Effects:
Yes, forms tumors in nude mice.
Gene expression:
Immunoglobulin(Surface And Secreted)
Growth Properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Lymphoblast-like
Receptor expression:
Interleukin 4(IL-4); low affinity IgE(CD23)
Split Ratio:
3x10^5-5x10^5 cells/mL
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:3-1:5.
Tissue:
Ascites; derived from metastatic site: peritoneum
Tumorigenic:
Yes

Documents

References

  • https://www.nature.com/articles/s41467-025-67093-w
  • https://www.nature.com/articles/s41467-024-50064-y
  • https://link.springer.com/article/10.1186/s12974-023-02848-0