BV2 Cell Line

BSL-2

Transformed cell line

Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase

MEM(PM150410)+10% FBS+1% P/S(PB180120)

Semi-adherent and semi-suspension

Atmosphere: Air, 95%; CO2, 5%Temperature: 37°C

37°C

Every 2 to 3 days

Epithelial-like

1:2-1:4

1:2 to 1:4

Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.

Brain; Microglia