Elabscience

MCF 10A Cell Line

Product Code:
CL-0525
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
36 years
Applications:
Transfection.
Background:
The MCF 10A cell line is a non-tumorigenic epithelial cell line.The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen.They exhibit three dimensional growth in collagen, and form domes in confluent cultures.Thus far, the cells have shown no signs of terminal differentiation or senescence.The line is responsive to Insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor(EGF).By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells.They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies.The calcium content of the medium exerts a strong effect on the morphology of the cells.
Biosafety Level:
1
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture medium:
DMEM/F12(PM150312)+5% HS+20ng/ml EGF+0.5ug/ml Hydrocortisone+10ug/ml Insulin+1% NEAA(PB180424)+1% P/S(PB180120)
Effects:
No, in immunosuppressed mice; Yes, in semisolid medium.
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%Temperature: 37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Epithelial
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Mammary gland, breast
Tumorigenic:
No

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