Procell system

KYSE-410 cell line

Product Code:
CL-0586
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
male, 51 years
Background:
This cell line was established from the poorly differentiated invasive esophageal squamous cell carcinoma from the cervical esophagus of a 51-year-old Japanese male prior to treatment (tumor invasion into the adventitia was obvious). The cells were reported to have an overexpression of heparin binding growth factor (hst-1) and cyclin D.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture medium:
RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Doubling Time:
~32-45 hours
Growth Properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:4-1:6
Subcultivation Ratio:
1:4 to 1:6
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Esophagus

Documents

References

  • https://aacrjournals.org/cancerdiscovery/article/doi/10.1158/2159-8290.CD-24-0006/745202
  • https://link.springer.com/article/10.1186/s12943-025-02302-0
  • https://aacrjournals.org/cancerres/article/85/14/2694/763439