Procell system

HAL-01 cell line

Product Code:
CL-0588
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
female, 17 years
Background:
This cell line was established in 1990 from the peripheral blood of a 17-year-old female with acute lymphoblastic leukemia (ALL-L2). The cell line contains the fusion gene of t(17; 19)(q22; p13) TCF3-HLF (E2A-HLF).
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Doubling Time:
~48 hours
Growth Properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Lymphoblast-like
Split Ratio:
5x10^5-1x10^6 cells/mL
Subcultivation Ratio:
1:2 to 1:3
Subculturing:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:3.
Tissue:
Peripheral blood

Documents

References

  • https://link.springer.com/article/10.1186/s12885-023-10589-9
  • https://www.sciencedirect.com/science/article/pii/S0161589023000986