Procell system

Daoy cell line

Product Code:
CL-0615
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
Male,4 years
Background:
The Daoy cell line was established in 1985 by P. F Jacobsen of the Royal Perth Hospital in Western Australia.The line was derived from biopsy material taken from a tumor in the posterior fossa of a 4 year old boy.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage Temperature: Liquid Nitrogen Vapor Phase
Culture medium:
MEM(PM150410)+10% FBS+1%P/S(PB180120)
Doubling Time:
~33.6 hours (PubMed=2993532); ~34 hours (ATCC)
Growth Properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Polygonal
Split Ratio:
1:3-1:6
Subcultivation Ratio:
1:2 to 1:5
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Brain

Documents

References

  • https://spj.science.org/doi/full/10.34133/bmr.0237
  • https://onlinelibrary.wiley.com/doi/abs/10.1002/mc.70016
  • https://link.springer.com/article/10.1007/s12032-023-01947-5