Elabscience

PC 61 5.3 [PC 61; PC 61.5.3] Cell Line

Product Code:
CL-0663
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Mouse
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Background:
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Biosafety Level:
1
Cryopreservation:
Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
DMEM(PM150210)+10%FBS+1%P/S(PB180120)
Gene expression:
immunoglobulin, monoclonal antibody, against mouse interleukin-2 (interleukin 2, IL-2) receptor (CD25)
Growth properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Medium Renewal:
Add fresh medium every 2 to 4 days
Morphology:
Lymphoblast
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 105 cells/mL and maintain between 1 x 105 and 5 x 105 cells/mL.
Tissue:
Spleen

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