Procell system

NB4 Cell Line

Product Code:
CL-0676
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
Female, 23 years
Background:
established from the bone marrow of a 23-year-old woman with acute promyelocytic leukemia (APL = AML FAB M3) in second relapse in 1989; a method of NB-4 transfection was patented in 2016; cells carry the t(15;17) PML-RARA fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze Medium: 55% Basal Medium+40% FBS+68% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
RPMI-1640 (PM150110)+10% FBS+1% P/S (PB180120)
Doubling Time:
~35-45 hours
Growth Properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Lymphoblast-like
Split Ratio:
1:2-1:3
Tissue:
Bone marrow

Documents

References

  • https://www.cell.com/cell/abstract/S0092-8674(25)01499-0
  • https://link.springer.com/article/10.1186/s12943-024-02013-y
  • https://www.nature.com/articles/s41467-025-66931-1