Procell system

D341Med Cell Line

Product Code:
CL-0683
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
3.5 years
Background:
The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
MEM(PM150410)+10%FBS+1mM Sodium Pyruvate+1%P/S(PB180120)
Gene expression:
glutamine synthetase positive; neuron specific enolase positive; glial fibrillary acidic proteins negative; S100 (S-100) protein negative; neuroectodermal antigen positive, recognized by the UJ13A monoclonal antibody
Growth Properties:
Aggregated suspension with loose adherence
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Lymphoblast-like
Split Ratio:
1:2-1:4
Subcultivation Ratio:
1:3 to 1:5
Tissue:
Brain
Tumorigenic:
Yes

Documents

References

https://spj.science.org/doi/full/10.34133/bmr.0237