Procell system

SU-DHL-4 Cell Line

Product Code:
CL-0692
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
38 years
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
RPMI-1640(PM150110)+10%FBS+1%P/S(PB180120)
Doubling Time:
~40 hours
Gene expression:
IgG+; Kappa+; IgM-; IgA-; IgD-; Lambda-; This cell line has relatively high expression levels of Bax; Bak; AIF; high caspase-9 activity.
Growth Properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 2 to 4 days
Morphology:
Lymphoblast-like
Split Ratio:
5x10^5-1x10^6 cells/mL
Subcultivation Ratio:
1:2 to 1:6
Subculturing:
Cultures can be maintained by the addition of fresh medium. An inoculum of 1.0 x 105to 2.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 8.0 x 105 and 1.0 x 106 cells/mL.
Tissue:
Peritoneal effusion

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S0891584924005318
  • https://pubs.acs.org/doi/abs/10.1021/acs.analchem.4c06244
  • https://link.springer.com/article/10.1007/s00262-023-03526-z