Procell system

NALM-6 Cell Line

Product Code:
CL-0701
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
19 years
Background:
Established from the peripheral blood of a 19-year-old man with acute lymphoblastic leukemia (ALL) in relapse in 1976. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
RPMI-1640(PM150110)+10%FBS+1%P/S(PB180120)
Doubling Time:
~25 hours (PubMed=25984343); ~19 hours (PubMed=20922763); ~40.2 hours (PubMed=8847894); ~36 hours (DSMZ)
Growth Properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
Every 3 days
Morphology:
Lymphoblast-like
Split Ratio:
1:2-1:3
Subcultivation Ratio:
1:2 to 1:3
Subculturing:
seed out at ca. 1 x 106 cells/ml; maintain at 1.0-2.0 x 106 cells/ml; split ratio of 1:2 to 1:3 every 3 days; minimum density of 0.5 x 106 cells/ml
Tissue:
Peripheral blood

Documents

References

  • https://www.cell.com/cell/fulltext/S0092-8674(25)00910-9
  • https://www.cell.com/trends/biotechnology/fulltext/S0167-7799(26)00229-5
  • https://www.nature.com/articles/s41419-024-06599-5