Procell system

Capan-2 Cell Line

Product Code:
CL-0709
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
56 years
Antigen expression:
Blood Type B; Rh+
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze Medium: 55% Basal Medium+40% FBS+5% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture medium:
McCoy's 5A(PM150710)+10%FBS+1%P/S(PB180120)
Doubling Time:
~96 hours (PubMed=3019537); ~45-60 hours (CLS); ~50-70 hours (DSMZ)
Gene expression:
Blood Type B; Rh+; high levels of MUC-1 mucin mRNA; low levels of MUC-2 mRNA; MUC-3 gene not expressed
Growth Properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Epithelial-like
Split Ratio:
1:2-1:4
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Pancreas
Tumorigenic:
Yes

Documents

References

  • https://jeccr.biomedcentral.com/articles/10.1186/s13046-022-02310-8
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9630940/
  • https://pubs.acs.org/doi/abs/10.1021/acs.jmedchem.4c02961