Procell system

SW1463 Cell Line

Product Code:
CL-0742
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
Female,66Y
Antigen expression:
Blood type A; Rh +
Background:
SW1463 is a cell line exhibiting epithelial morphology that was isolated from the rectum of a White, 66-year-old, female patient with colorectal adenocarcinoma. This cell line was deposited by A Leibovitz and can be used in cancer research.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freeze Medium: 55% Basal Medium+40% FBS+84% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
Leibovitz's L-15 (PM151010)+10% FBS+1% P/S (PB180120)
Doubling Time:
~3.5 days
Gene expression:
carcinoembryonic antigen (CEA) 223 ng/106 cells/10 days; keratin
Growth Properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 100%; Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:2-1:4
Subcultivation Ratio:
1:3 to 1:4
Tissue:
Large intestine; Rectum
Tumorigenic:
Yes; Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S0014482723002811
  • https://pmc.ncbi.nlm.nih.gov/articles/PMC12484439/