Procell system

MDBK [NBL-1]

Product Code:
CL-0153
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Bovine (Cattle)
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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Further Information

Age Gender:
Adult;Male
Background:
MDBK (NBL-1) cells were established by S. H. Madin and N. B. Darby on February 18, 1957, from a visually normal adult cow kidney. In 1973, this MDBK (NBL-1) cell line was infected with bovine viral diarrhea virus (BVDV); in 1982, a strain of MDBK (NBL-1) cells that hadn't been infected with BVDV was identified, originally from a freezing vial of the 96th generation from ATCC in July 1967. The descendants of these MDBK (NBL-1) cells were tested by NVSL, and no evidence of viral contamination was found. By August 1982, when R. A. Van Deusen provided MDBK (NBL-1) to ATCC, the cells had been passaged to the 110th generation and were not infected with BVDV.
Biosafety Level:
BSL-2
Cell Type:
spontaneously immortalized cell line
Cryopreservation:
Cryopreservation solution: serum-free, non-programmed cryopreservation solution [PB180438]
Culture medium:
RPMI-1640 [PM150110]+10% FBS [164210]+1% P/S [PB180120]
Doubling Time:
~35 hours
Gene expression:
keratin
Growth Properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:2-1:4
Subcultivation Ratio:
1:2 to 1:4
Tissue:
kidney

Documents

References

  • https://www.nature.com/articles/s44161-025-00665-7
  • https://www.nature.com/articles/s41541-024-00846-8
  • https://www.sciencedirect.com/science/article/pii/S156757692600723X