Elabscience

MDBK [NBL-1]

Product Code:
CL-0153
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Bovine (Cattle)
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
Adult;Male
Background:
MDBK (NBL-1) cells were established by S. H. Madin and N. B. Darby on February 18, 1957, from a visually normal adult cow kidney. In 1973, this MDBK (NBL-1) cell line was infected with bovine viral diarrhea virus (BVDV); in 1982, a strain of MDBK (NBL-1) cells that hadn't been infected with BVDV was identified, originally from a freezing vial of the 96th generation from ATCC in July 1967. The descendants of these MDBK (NBL-1) cells were tested by NVSL, and no evidence of viral contamination was found. By August 1982, when R. A. Van Deusen provided MDBK (NBL-1) to ATCC, the cells had been passaged to the 110th generation and were not infected with BVDV.
Biosafety Level:
BSL-2
Cryopreservation:
Cryopreservation solution: serum-free, non-programmed cryopreservation solution [PB180438]
Culture medium:
RPMI-1640 [PM150110]+10% FBS [164210]+1% P/S [PB180120]
Gene expression:
keratin
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelioid
Subcultivation Ratio:
1:2 to 1:4
Tissue:
Kidney; normal cells

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