Elabscience

bEnd.3 [BEND3]

Product Code:
CL-0598
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Mouse
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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Further Information

Age Gender:
6W;Sex unspecified
Antigen expression:
ICAM-1 +; VCAM-1 +; MAdCAM-1 +
Background:
The mouse brain vascular endothelial tumor cell bEnd.3 [BEND3] was isolated from the brain tissue of endothelial tumor mice and immortalized through retroviral transformation carrying the polyomavirus middle T antigen. This cell exhibits endothelial characteristics like expressing von Willebrand factor and uptaking low-density lipoprotein (LDL). The cells constantly express intercellular adhesion molecule 1 (ICAM-1), which increases after treatment with LPS, IL-1, and TNF-alpha. Earlier generations of bEnd.3 cells express MAdCAM-1 on the surface without stimulation, but after 30 passages they no longer do. Similarly, cells before passage 30 continuously express vascular cell adhesion molecule 1 (VCAM-1), but this stops after 30 passages. Tumor necrosis factor-alpha (TNF-alpha) can induce bEnd.3 cells to express P-selectin, with stronger expression after 30 passages. bEnd.3 cells can be used in neuroscience research.
Biosafety Level:
BSL-1
Cryopreservation:
Cryopreservation solution: General serotype program cryopreservation solution [PB180436]
Culture medium:
DMEM [PM150210]+10% FBS [164210]+1% P/S [PB180120]
Gene expression:
von Willebrand factor,ICAM-1 +; VCAM-1 +; MAdCAM-1 +,The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL). The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd3.
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelioid
Subcultivation Ratio:
1:2-1:4
Tissue:
Cerebral cortex

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