Elabscience

HePa1-6-GFP

Product Code:
CL-1006
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Mouse
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
Female
Background:
Hepa1-6-GFP is a cell line derived from BW7756 hepatoma induced in a C57L mouse. It expresses AFP, α1-antitrypsin, and amylase, and tests negative for ectromelia virus. The cells can be propagated in serum-free medium consisting of 75% DMEM and 25% Waymouth?s MAB 87/3, supplemented with 3x10^-8 M selenium. For drug screening: Fluorescent gene fragments are integrated into the cellular genome via lentiviral infection, enabling the cells to express fluorescent proteins that can be visualized under a fluorescence microscope, allowing easy tracking and detection of labeled cells. Owing to variable fluorescence expression resulting from lentiviral transduction, antibiotic selection may be used to enrich for cells with high fluorescent protein expression. During routine culture, supplemental selection is recommended at a final concentration of 4 ug/mL puromycin, applied 2?3 times per month or at a customized frequency. A single round of supplemental selection is also advised following thawing of cryopreserved cells. Daily addition of puromycin during routine culture is not required.
Biosafety Level:
BSL-2
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
DMEM [PM150210]+10% Nutrient+1% Supplement
Dissociation Duration:
2-3 min
Growth properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Subcultivation Ratio:
1:3-1:6
Tissue:
Liver

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