Procell system

Eca-109 Cell Line

Product Code:
CL-0077
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
Female
Background:
The Eca-109 cell line was established in 1973 by primary explant culture of esophageal squamous cell carcinoma tissue from the mid-thoracic portion of the esophagus. Eca-109 cells form tumors after transplantation into BALB/c nude mice.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Dissociation Duration:
2-3 min
Doubling Time:
~28 hours
Growth Properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:2-1:4
Subcultivation Ratio:
1:2-1:4
Tissue:
Esophagus
Tumorigenic:
Yes, in nude mice.

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S1368764625001426
  • https://jitc.bmj.com/content/12/7/e008888.abstract
  • https://www.sciencedirect.com/science/article/pii/S0304383523003919