Procell system

H4 Cell Line

Product Code:
CL-0087
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
37Y, Male
Background:
The H4 cell line was established in 1973. It was derived from brain tissue of a 37-year-old patient with glioma. The tumorigenic properties of H4 cells are suppressed; generally, no tumor nodules form when the cells are inoculated into animals. H4 cells possess the ability to repair MNNG-damaged adenovirus type 5.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
DMEM [PM150210]+10% Nutrient+1% Supplement
Dissociation Duration:
2-3 min
Growth Properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:3-1:4
Subcultivation Ratio:
1:3-1:4
Tissue:
Brain
Tumorigenic:
No, in immunosuppressed mice. Yes, in semisolid medium.

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S1043661823003353
  • https://www.nature.com/articles/s41420-024-02105-0
  • https://www.sciencedirect.com/science/article/pii/S0898656824001050