Elabscience

LNCaP clone FGC Cell Line

Product Code:
CL-0143
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
50Y, Male
Background:
LNCaP clone FGC is a human prostate cancer cell line isolated via needle aspiration biopsy from the left supraclavicular lymph node of a 50-year-old Caucasian male with blood type B+, who suffered from metastatic prostate carcinoma. LNCaP clone FGC cells respond to 5-alpha-dihydrotestosterone; this androgen modulates cellular proliferation and stimulates acid phosphatase secretion. LNCaP clone FGC cells do not grow as a homogeneous monolayer but form cell clusters, which can be dissociated by repeated gentle pipetting during subculturing. The cells exhibit weak substrate adhesion, cannot reach full confluence, and rapidly acidify culture medium. LNCaP clone FGC proliferates extremely slowly, so cultures should remain undisturbed within the initial 48 hours post-passage. Most LNCaP clone FGC cells detach from the flask surface and float in medium during flask transportation. Upon receiving the shipped flask, incubate it under standard monolayer culture conditions for 24?48 hours to facilitate cell reattachment, followed by medium replacement with fresh culture medium. If necessary, collect all cell suspension from the flask, centrifuge at 300 ? g for 15 minutes, resuspend the cell pellet in 10 mL complete medium, and seed into a single culture flask.
Biosafety Level:
BSL-1
Cryopreservation:
Freezing Medium (Serum-free & animal origin-free) [PB180438]
Culture medium:
RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Dissociation Duration:
2-3 min
Gene expression:
human prostatic acid phosphatase; prostate specific antigen
Growth properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Subcultivation Ratio:
1:3-1:4
Tissue:
Prostate; derived from metastatic site: left supraclavicular lymph node
Tumorigenic:
Yes, in soft agar.Yes, the cells are tumorigenic in nude mice.

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