Procell system

WI-38 Cell Line

Product Code:
CL-0243
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
16FW, Female
Background:
The WI-38 cell line was derived from normal embryonic lung tissue obtained from a 3-month-gestation fetus. As the first human diploid cell line used for human vaccine preparation, WI-38 cells can be promoted to grow by adding TNF-α to the culture medium.
Biosafety Level:
BSL-1
Cell Type:
Finite cell line
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
MEM, with NEAA [PM150410]+10% Nutrient+1% Supplement
Dissociation Duration:
1-2 min
Growth Properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Fibroblast-like
Split Ratio:
01:02
Subcultivation Ratio:
1~2
Tissue:
Lung

Documents

References

  • https://www.nature.com/articles/s41392-025-02454-x
  • https://pmc.ncbi.nlm.nih.gov/articles/PMC12223780/
  • https://www.sciencedirect.com/science/article/pii/S0304383520301841