Procell system

HPAC Cell Line

Product Code:
CL-0361
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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  • Further Information
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Further Information

Age Gender:
64Y, Female
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freezing Medium (Serum-free & animal origin-free) [PB180438]
Culture medium:
DMEM/F12 [PM150312]+0.5% Supplement1+40 ng/mL Supplement2+10 ng/mL Supplement3+5% Nutrient+1% Supplement4
Dissociation Duration:
3-5 min
Doubling Time:
~23-44 hours
Gene expression:
HPAC cells are positive for keratin and negative for vimentin and chromogranin A.
Growth Properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Receptor expression:
epidermal growth factor (EGF), expressed; glucocorticoid, expressed; epidermal growth factor (EGF); glucocorticoid
Split Ratio:
1:3-1:6
Subcultivation Ratio:
1:3-1:6
Tissue:
Pancreas
Tumorigenic:
Yes, the cells form tumors in athymic nude mice at the site of inoculation which are histologically similar to the tumor of origin. Yes, the cells have a colony forming efficiency of 64% on plastic and 3.2% in agarose.

Documents

References

  • https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202206912
  • https://pubs.acs.org/doi/abs/10.1021/acs.jmedchem.6c00603
  • https://pmc.ncbi.nlm.nih.gov/articles/PMC12197932/