Procell system

SW48 Cell Line

Product Code:
CL-0513
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
82Y, Female
Antigen expression:
HLA A32, A33, B27, B35; Blood type AB; Rh+
Background:
SW48 were cells isolated from the large intestine of a 82-day-old, White, female grade IV Dukes C colorectal cancer patient, and can be used in cancer research. This cell line is a suitable transfection host.
Biosafety Level:
BSL-1
Cell Type:
Cancer cell line
Cryopreservation:
Freezing Medium (Serum-free & animal origin-free) [PB180438]
Culture medium:
Leibovitz's L-15 [PM151010]+10% Nutrient+1% Supplement
Dissociation Duration:
2-3 min
Doubling Time:
~33 hours (PubMed=25984343)
Gene expression:
carcinoembryonic antigen (CEA) 0.6 ng/10^6 cells/10 days; keratin
Growth Properties:
Adherent
Incubation Atmosphere:
Air, 100%, 37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Split Ratio:
1:2-1:4
Subcultivation Ratio:
1:2-1:4
Tissue:
Large intestine; Colon
Tumorigenic:
Yes; Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells).

Documents

References

  • https://jeccr.biomedcentral.com/articles/10.1186/s13046-025-03277-y
  • https://www.nature.com/articles/s41419-024-07057-y
  • https://www.hindawi.com/journals/jo/2022/7905787/