Elabscience

NK-92 Cell Line

Product Code:
CL-0530
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
50Y, Male
Background:
The NK-92 cell line is an IL-2-dependent NK cell line derived from peripheral blood mononuclear cells of a 50-year-old Caucasian male with rapidly progressive non-Hodgkin's lymphoma. NK-92 cells are cytotoxic to a wide range of malignant cells; chromium release assays have demonstrated their ability to kill both K562 cells and Daudi cells. NK-92 cells (after irradiation to prevent proliferation) can be effectively used for immunological ex vivo purging of leukemia from blood without compromising hematopoietic cell function. NK-92 cells exhibit the following characteristics: positive for the surface markers CD2, CD7, CD11a, CD28, CD45, and CD54; negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34, and HLA-DR.
Biosafety Level:
BSL-2
Cryopreservation:
Freezing Medium (Serum-free & animal origin-free) [PB180438]
Culture medium:
MEMα [PM150422]+0.2 mM Inositol+0.1 mM Supplement1+0.02 mM Folic Acid+IL-2 [PB180634]+12.5% Nutrient2+12.5% Nutrient1+1% Supplement2
Growth properties:
Suspension
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Lymphoblast-like
Subcultivation Ratio:
5x10^5-1x10^6 cells/mL
Tissue:
Peripheral blood

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