Elabscience

NK-92MI Cell Line

Product Code:
CL-0533
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

No additional charges, what you see is what you pay! *

Show prices   How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: China.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Related Products
  • Show All

Further Information

Age Gender:
50Y, Male
Background:
The NK-92 cell line is an interleukin-2 (IL-2)-dependent natural killer (NK) cell line derived from peripheral blood mononuclear cells of a 50-year-old Caucasian male with rapidly progressive non-Hodgkin's lymphoma. The NK-92MI cell line is an IL-2-independent NK subline generated from NK-92 cells via retroviral transduction. Parental NK-92 cells were transduced with human IL-2 cDNA carried by the retroviral MFG-hIL-2 vector; transduction was stable, presumably due to integration of the vector into the host genome. NK-92 cells are cytotoxic to a wide range of malignant cells; chromium-release assays confirm their ability to kill K562 and Daudi cells. NK-92 cells exhibit the following immunophenotypic characteristics: positive for surface markers CD2, CD7, CD11a, CD28, CD45, and CD54; negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34, and HLA-DR. The parental IL-2-dependent NK-92 cell line and a second IL-2-independent subline, NK-92CI, are available from the ATCC. Both NK-92MI and NK-92CI variants contain and express the hIL-2 cDNA and secrete bioactive IL-2; NK-92MI cells produce higher levels of IL-2 than NK-92CI cells, while parental NK-92 cells do not produce IL-2. A culture submitted to ATCC in September 1998 was found to be contaminated with mycoplasma; the progeny were cured of mycoplasma via 21-day treatment with BM Cyclin. Six weeks post-treatment, mycoplasma testing via Hoechst staining, PCR, and standard culture all yielded negative results.
Biosafety Level:
BSL-2
Cryopreservation:
Freezing Medium (Serum-free & animal origin-free) [PB180438]
Culture medium:
MEMα [PM150422]+0.2 mM Inositol+0.1 mM Supplement1+0.02 mM Folic Acid+12.5% Nutrient2+12.5% Nutrient1+1% Supplement2
Growth properties:
Suspension
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Lymphoblast-like
Subcultivation Ratio:
5x10^5-1x10^6 cells/mL
Tissue:
Peripheral blood

Documents