Procell system

HMEC-1 Cell Line

Product Code:
CL-0576
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
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Further Information

Age Gender:
<1M, Male
Background:
The HMEC-1 cell line is a human microvascular endothelial cell line isolated from the foreskin of a male patient. The cells were subsequently transfected with the pSVT vector, after which they retained many endothelial cell characteristics and were established as an immortalized cell line through continuous passaging. As an immortalized cell line, HMEC-1 provides a continuously renewable source of human microvascular endothelial cells and can be used to construct in vitro models of human dermal microvascular endothelial cells for various research applications. These cells are applicable to studies on angiogenesis, leukocyte trafficking, wound healing, inflammation, circulation, tumor growth and metastasis, cancer research, endothelial function, cell trafficking, surface molecule interactions, drug screening, and toxicological studies in the pharmaceutical and cosmetic industries.
Biosafety Level:
BSL-2
Cell Type:
Transformed cell line
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
Complete Growth Medium for HMEC-1 [CM-0576]
Dissociation Duration:
2-3 min
Doubling Time:
~48 hours
Growth Properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Endothelial-like
Split Ratio:
1:2-1:3
Subcultivation Ratio:
1:2-1:3
Tissue:
Foreskin; Skin; Dermis

Documents

References

  • https://www.nature.com/articles/s41467-022-33475-7
  • https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.202201705
  • https://onlinelibrary.wiley.com/doi/abs/10.1002/smsc.202500046