Procell system

MT-4 Cell Line

Product Code:
CL-0655
Product Group:
Cell Lines
Supplier:
Procell system
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation cryovials should be stored in liquid nitrogen at ?150°C to ?196°C. Storage at ?80°C is restricted to short-term interim use only.
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
<1D, Male
Background:
MT-4 is an adult T-cell leukaemia cell line derived from a 50-year-old Japanese male presenting with generalized lymphadenopathy and hepatosplenomegaly, established via co-culture of the patient?s peripheral leukocytes with male umbilical cord lymphocytes. The cells harbour HTLV-1 and support HIV propagation. Manipulation of this cell line requires Biosafety Level 3 containment facilities.
Biosafety Level:
BSL-2
Cell Type:
Transformed cell line
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Doubling Time:
~30 hours
Growth Properties:
Suspension
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Incubation Temperature:
37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Lymphoblast-like
Split Ratio:
3x10^5-5x10^5 cells/mL
Subcultivation Ratio:
3x10^5-5x10^5 cells/mL
Tissue:
Peripheral blood

Documents

References

  • https://www.nature.com/articles/s41467-022-32104-7
  • https://www.science.org/doi/abs/10.1126/sciadv.adw7471
  • https://www.cell.com/cell-reports-medicine/fulltext/S2666-3791(24)00660-8