Elabscience

U-2 OS-GFP Cell Line

Product Code:
CL-0689
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

No additional charges, what you see is what you pay! *

Show prices   How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: China.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Related Products
  • Show All

Further Information

Age Gender:
15Y, Female
Background:
The U-2 OS cell line (originally named 2T) was established in 1964 by J. Pont?n and E. Saksela from a moderately differentiated sarcoma of the tibia in a 15-year-old female. When co-cultured with WI-38 cells or tested by complement fixation (CF) assay for simian virus 40 (SV40), respiratory syncytial virus (RSV), or adenoviruses, no viruses were detected. U-2 OS cells express insulin-like growth factor I (IGF-I) and II (IGF-II) receptors, as well as osteosarcoma-derived growth factor (ODGF). The U-2 OS-GFP cell line was constructed from U-2 OS cells by lentiviral transduction and is a stable cell line capable of long-term, high-level GFP expression. This cell line exhibits low passage, high viability, and good condition, making it suitable for experiments including flow cytometry and fluorescence imaging. Selection drug concentration: Puro = 2.0 ug/mL.
Biosafety Level:
BSL-2
Cryopreservation:
Freezing Medium (Serum-free & animal origin-free) [PB180438]
Culture medium:
McCoy's 5A [PM150710]+10% Nutrient+1% Supplement
Dissociation Duration:
2-3 min
Growth properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Subcultivation Ratio:
1:3-1:4
Tissue:
Bone

Documents