Elabscience

SW620-LUC Cell Line

Product Code:
CL-0700
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
51Y, Male
Antigen expression:
Blood Type A; Rh+
Background:
The SW620-LUC cell line was established from SW620 cells by lentiviral transduction, enabling stable expression of luciferase.
Biosafety Level:
BSL-1
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
DMEM [PM150210]+10% Nutrient+1% Supplement
Dissociation Duration:
2-3 min
Gene expression:
Carcinoembryonic antigen (CEA) 0.15 ng/10^6 cells/10 days; transforming growth factor alpha; matrilysin. The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative. The cells are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.
Growth properties:
Adherent
Incubation Atmosphere:
Air, 95%; CO2, 5%,37°C
Medium Renewal:
every 2 to 3 days
Morphology:
Epithelial-like
Subcultivation Ratio:
1:3-1:6
Tissue:
Large intestine; Colon; derived from metastatic site: lymph node
Tumorigenic:
Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1x10^7 cells).

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